THE BIURET REACTION
نویسندگان
چکیده
منابع مشابه
Quantitation of sputum protein by use of the biuret reaction.
A simple, accurate method is described for quantitatively determining the protein content of sputum by use of the biuret reaction. Fitteen mechanically homogenized specimens were examined with the biuret reagent described by Rosenthal and Cundiff, and the results compared with those obtained by the microKjeldahl technique. Over a range of 542 to 1917 mg of protein per 100 ml, the techniques cor...
متن کاملDetermination of serum proteins by means of the biuret reaction.
In the course of an investigation of the biochemical changes following experimental liver injury we felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum. Among the simpler procedures known, the biuret reaction seemed to offer the most encouraging possibilities. Variations and improvements in the application of the biuret reaction ...
متن کاملThe Biuret Reaction of Proteins in the Presence of Ethylene Glycol
The biuret method offers some advantages as a simple, rapid photometric method for the determination of proteins. In the methods which have been proposed, precipitation of cupric hydroxide gives rise to certain difficulties (1, 2). It occurred to us that the precipitates might be avoided by the addition of a reagent which would form a compound with copper which was soluble in alkali, but of suc...
متن کاملDetermination of total urinary protein by combined gel filtration and automated biuret reaction.
Determination of total urinary protein is now more frequently requested of the clinical chemistry laboratory, one reason being the increased number of patients with renal transplants for whom total urinary protein is determined as an index of immunological rejection (1). Available manual methods are cumbersome and inefficient under clinical laboratory conditions. Concentrations for total urinar...
متن کاملUrea and Biuret for Lambs
C ORN silage is a major source of roughage for ruminants in the cornbelt, because it is one of the most productive crops of total digestible nutrients and animal gains per acre. However, to make up a complete growingfinishing ration with corn silage it is necessary to supplement it with sources of energy, nitrogen and minerals. The addition of urea to corn, sorghum or grass at ensiling has give...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1928
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)83892-3